QuantCyte™ quantitation controls are precision engineered synthetic cell mimics designed to quantify antigen molecules per cell and support consistent expression benchmarking across research and development workflows. Each kit includes multiple defined antigen density levels that generate distinct fluorescence intensities, enabling accurate calibration and direct measurement of antibody binding capacity without requiring knowledge of fluorophore molecules per antibody.
Measured intensities can be incorporated into the provided Antigen Density Calculator to generate a signal to antigen standard curve, supporting quantitative readouts on cell targets using the same instrumentation and antibody. Unlike biospecimen‑derived materials, QuantCyte quantitation controls provide stable antigen presentation and eliminate the variability associated with donor cells. These lyophilized synthetic cell mimics are available in CD19 and can be custom engineered for additional targets to support diverse assay requirements.
All QuantCyte products are intended for research use only and are not suitable for diagnostic or therapeutic procedures. These products are safe to use in any environment, contain no biohazardous material and require no special disposal.
Find the latest controls for Functional CAR T Benchmarking, Spectral Unmixing & Compensation, Immunophenotyping Controls, Absolute Antigen Quantification and Instrument Standardization & Harmonization.
FlowCytes® WBC are a synthetic polymer based cell mimic with defined scatter populations designed to imitate lymphocyte, monocyte and granulocyte populations to support standardization and reproducibility of scatter settings across assays. Available in both Fixed and Unfixed formats to accommodate fixed or viable cell workflows.
StimCytes™ Activation Reagents are precision engineered synthetic cell mimics designed to support CAR‑T potency assessment through targeted activation, cytokine release and biomarker driven assay development. Each kit includes matched positive and negative cell mimics that reflect baseline and enhanced activation profiles, enabling functional readouts that mirror the behavior of engineered T cells during antigen engagement.
Unlike cell controls, StimCytes Activation Reagents provide consistent antigen expression and reproducible activation profiles that remove the variability associated with donor‑derived materials. These lyophilized synthetic cells are available in CD19, BCMA and CD20 formats and are designed to streamline assay optimization with full technical support. BCMA and CD20 StimCytes Controls are part of an early release program with specifications subject to refinement as additional validation is completed.
All StimCyte products are intended for research use only and are not suitable for diagnostic or therapeutic procedures. These products are safe to use in any environment, contain no biohazardous material and require no special disposal.
Biomarker & Gating Controls are a family of lyophilized cell mimics engineered for reliable performance checks and normalization controls in immunophenotyping and flow cytometry workflows. Each product features physiologically relevant antigen densities and engineered scatter characteristics that closely resemble human immune cell populations, enabling confident population discrimination and gating across diverse cytometry platforms. The TruCytes portfolio includes controls designed to provide positive and negative signal detection for key biomarkers targeted by specific antibodies, supporting enumeration and viability assessment of pan‑lymphocyte, T cell subset, and stem cell populations.
Offering superior lot‑to‑lot reproducibility and exceptional long‑term stability, Biomarker & Gating Controls outperform fresh primary cells, frozen preparations and conventional lyophilized products. With no biohazard material and no special handling or disposal requirements, they provide dependable alternatives to biologically derived controls while maintaining consistent antigen presentation and physiologically relevant scatter profiles. Their lyophilized format ensures consistent reconstitution and extended shelf life, supporting multi‑site standardization, training and long‑term assay harmonization.
Together, the Biomarker & Gating Controls family delivers a scalable and reproducible solution for accurate gating, antibody performance assessment and instrument verification across daily QC, assay validation and longitudinal performance monitoring. All TruCytes products are intended for research use only and are not suitable for diagnostic or therapeutic procedures. These products are safe to use in any environment, contain no biohazardous material and require no special disposal.
Slingshot's Instrument Setup and Harmonization Controls are precision engineered synthetic hydrogel reference particles designed to support instrument‑to‑instrument standardization of forward and side scatter. Each control includes multiple defined scatter populations that span a biologically relevant range, enabling consistent calibration, linearity assessment and long‑term performance tracking across diverse flow cytometry platforms.
These controls provide stable reproducible scatter characteristics that eliminate the variability associated with biologically derived materials. ScatterBridge® products can be used to align scatter settings between instruments, optimize FSC and SSC detection and recalibrate scatter following major instrument service. FlowCytes® products can be used individually or in tandem with ScatterBridge to ensure reproducbility in scatter settings across assays.
As synthetic, non-biohazardous materials, they require no special handling or disposal.
PhenoCytes™ are a family of lyophilized cell mimics engineered for reliable performance checks and normalization controls in immunophenotyping and flow cytometry workflows. Each product features physiologically relevant antigen densities and engineered scatter characteristics that closely resemble human immune cell populations, enabling confident population discrimination and gating across diverse cytometry platforms. The TruCytes portfolio includes controls designed to provide positive and negative signal detection for key biomarkers targeted by specific antibodies, supporting enumeration and viability assessment of pan‑lymphocyte, T cell subset, and stem cell populations.
Offering superior lot‑to‑lot reproducibility and exceptional long‑term stability, PhenoCytes outperform fresh primary cells, frozen preparations and conventional lyophilized products. With no biohazard material and no special handling or disposal requirements, they provide dependable alternatives to biologically derived controls while maintaining consistent antigen presentation and physiologically relevant scatter profiles. Their lyophilized format ensures consistent reconstitution and extended shelf life, supporting multi‑site standardization, training and long‑term assay harmonization.
Together, the PhenoCytes family delivers a scalable and reproducible solution for accurate gating, antibody performance assessment and instrument verification across daily QC, assay validation and longitudinal performance monitoring. All PhenoCytes products are intended for research use only and are not suitable for diagnostic or therapeutic procedures. These products are safe to use in any environment, contain no biohazardous material and require no special disposal.
QuantCytes™ quantitation controls are precision-engineered synthetic cell mimics designed to quantify antigen molecules per cell and support consistent expression benchmarking across research and development workflows. Each kit includes multiple defined antigen density levels that generate distinct fluorescence intensities, enabling accurate calibration and direct measurement of antibody binding capacity without requiring knowledge of fluorophore molecules per antibody.
Measured intensities can be incorporated into the provided Antigen Density Calculator to generate a signal to antigen standard curve, supporting quantitative readouts on cell targets using the same instrumentation and antibody. Unlike biospecimen‑derived materials, QuantCytes™ quantitation controls provide stable antigen presentation and eliminate the variability associated with donor cells. These lyophilized synthetic cell mimics are available in CD19 and can be custom engineered for additional targets to support diverse assay requirements.
All QuantCytes™ products are intended for research use only and are not suitable for diagnostic or therapeutic procedures. These products are safe to use in any environment, contain no biohazardous material and require no special disposal.
ScatterBridge™ Controls are precision engineered synthetic hydrogel reference particles designed to support instrument‑to‑instrument standardization of forward and side scatter. Each control includes multiple defined scatter populations that span a biologically relevant range, enabling consistent calibration, linearity assessment and long‑term performance tracking across diverse flow cytometry platforms.
These controls provide stable reproducible scatter characteristics that eliminate the variability associated with biologically derived materials. ScatterBridge products can be used to align scatter settings between instruments, optimize FSC and SSC detection and recalibrate scatter following major instrument service.
As synthetic, non-biohazardous materials, they require no special handling or disposal.
SpectraComp® panel setup controls are precision-engineered cell mimics designed to enable accurate spectral unmixing and compensation in multiparameter flow cytometry workflows. These controls possess optical and autofluorescent properties similar to real cells. When stained with fluorescently conjugated antibodies, they generate positive and negative fluorescence peaks that match the behavior of biological samples.
Depending on the specific product, these single-stain controls capture antibody species across mouse, rat, hamster, rabbit, and human — covering a wide range of panel designs and fluorophore selections. Their consistent fluorescence intensity and cell-like scatter characteristics outperform polystyrene-based beads and reduce errors associated with spectral overlap.
The SpectraComp family supports reliable unmixing across instruments and panels, improving reference spectra and gating fidelity in multicolor experiments. It replaces bead-based single-color controls and variable cell-based controls — with no special handling or safety precautions required and validated on analytical flow cytometers for assay development and high-parameter panel design.
SpectraComp® and ViaComp® panel setup controls are precision engineered cell mimics with optical and autofluorescent properties similar to real cells. After staining with fluorescently conjugated antibodies, these controls generate positive and negative fluorescence peaks that closely match the behavior of biological samples. Each single‑stain control captures multiple antibody species, including mouse, rat, hamster, rabbit and human, ensuring broad compatibility across diverse panels and fluorophore selections.
Designed to reduce errors associated with spectral overlap, SpectraComp® controls provide consistent fluorescence intensity and cell‑like scatter characteristics that outperform traditional polystyrene‑based beads. Their cell‑mimic composition supports reliable unmixing across instruments and panels, improving reference spectra and gating fidelity in complex multicolor experiments. With these properties, SpectraComp and ViaComp controls serve as robust replacements for bead‑based single‑color controls and reduce reliance on variable cell‑based controls.
The Panel Setup family also includes ViaComp® viability controls, advanced 2‑in‑1 synthetic cell mimics that bind both DNA‑intercalating dyes and amine‑reactive viability dyes to replicate dead‑cell staining behavior. Together, these products deliver biologically relevant fluorescent signatures for dependable unmixing, compensation, viability modeling and instrument verification across assay development and high‑parameter panel design.
StimCytes™ Activation Reagents are precision engineered synthetic cell mimics designed to support CAR‑T potency assessment through targeted activation, cytokine release and biomarker driven assay development. Each kit includes matched positive and negative cell mimics that reflect baseline and enhanced activation profiles, enabling functional readouts that mirror the behavior of engineered T cells during antigen engagement.
Unlike cell controls, StimCytes Activation Reagents provide consistent antigen expression and reproducible activation profiles that remove the variability associated with donor‑derived materials. These lyophilized synthetic cells are available in CD19, BCMA and CD20 formats and are designed to streamline assay optimization with full technical support. BCMA and CD20 StimCytes Controls are part of an early release program with specifications subject to refinement as additional validation is completed.
All StimCyte products are intended for research use only and are not suitable for diagnostic or therapeutic procedures. These products are safe to use in any environment, contain no biohazardous material and require no special disposal.
Slingshot Biosciences Training Kits provide ready-to-use cell mimic panels that let you quickly train teams on flow cytometry workflows, instrument setup, and data analysis using stable, reproducible controls instead of fragile primary cells.
The ViaComp® family of viability controls are advanced 2-in-1 synthetic cell mimics designed to model dead-cell staining behavior in spectral and conventional flow cytometry workflows. These precision-engineered particles bind both DNA-intercalating dyes (such as 7-AAD and PI) and amine-reactive viability dyes, replicating the staining behavior of real cells.
ViaComp delivers consistent performance across operators and instruments. Their bright positive signals and negative control particles generate fluorescence peaks that enable accurate identification of live and dead cell populations. Because they are synthetic, they eliminate the variability, fragility, and preparation overhead associated with cell-based controls.
The ViaComp family ships ready-to-use and is offered in two configurations:
All ViaComp controls have a 24-month shelf life at -20°C and have been validated on analytical flow cytometers for instrument verification and assay development. For research use only.
